Disruption of Bacterial Spores by Superheating - a Method for Fast Dna Release
نویسندگان
چکیده
Bacterial spores are forms of bacteria that are capable of surviving in harsh conditions. We have investigated thermal lysis of the spores and subsequently release of their DNA. We were able to heat water-based samples at ambient pressure to temperatures far above 100 °C without boiling, known as superheating process. It resulted in the breaking of spores in a matter of seconds. Successful release of DNA from spores of the genus Bacillus was confirmed by real-time PCR (polymerase chain reaction). This process can be used for release DNA from Anthrax spores, allowing its presence to be detected.
منابع مشابه
Detection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR
American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...
متن کاملComparison of the utility of five commercial kits for extraction of DNA from Aspergillus fumigatus spores.
The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using A...
متن کاملMicrofluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis.
Biologic agent screening is a three-step process: lysis of host cell membranes or walls to release their DNA, polymerase chain reaction to amplify the genetic material and screening for distinguishing genetic signatures. Macrofluidic devices commonly use sonication as a lysis method. Here, we present a piezoelectric microfluidic minisonicator and test its performance. Eukaryotic human leukemia ...
متن کاملA minisonicator to rapidly disrupt bacterial spores for DNA analysis.
Concerns about the use of anthrax spores as a weapon of mass destruction have motivated the development of portable instruments capable of detecting and monitoring a suspected release of the agent. Optimal detection of bacterial spores by PCR requires that the spores be disrupted to make the endogenous DNA available for amplification. The entire process of spore lysis, PCR, and detection can ta...
متن کاملInduced release of dipicolinic acid from spores of Bacillus megaterium.
Conditions under which dipicolinic acid may be released from bacterial spores probably relate to the chemical state of the dipicolinic acid in the spore, to its physical location in the spore, and to its influence on the physiological properties of the spore. The latter aspect embraces the relation between release of dipicolinic acid and the viability of the spore, its heat resistance, and its ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012